Fig. 3: Endometrial and peripheral blood Treg have alternate characteristics.

a Transcriptomic analysis of Treg derived from endometrium (n = 5) versus peripheral blood (PB) (n = 5) from RPL patients, volcano plot depicting differential gene expression (DESeq2) between endometrial and PB Treg showing top 25 differentially expressed genes, (b) heatmap of top 30 upregulated (right panel) and downregulated (left panel) genes in the endometrium versus PB, red = high, blue = low expression, P = PRL (n = 3) and S = SRPL (n = 2) for endometrium (E) and peripheral blood (PB) Treg, (c) Flow cytometric analysis of matched peripheral blood (red bars) and endometrium (grey bars) Treg from patients with RPL, the mean fluorescence intensity (mfi) of FOXP3, HELIOS, CTLA4, TIGIT, IL18R (n = 10), CXCR6, ICOS (n = 3), LAG, PD1, CD39 and TIM3 (n = 4) was determined (bars = mean ± SEM; dots = individual cell sample).