Fig. 4: Treg characterisation in primary and secondary RPL reveals an altered transcriptome. | Mucosal Immunology

Fig. 4: Treg characterisation in primary and secondary RPL reveals an altered transcriptome.

From: Characterisation of peri-implantation endometrial Treg and identification of an altered phenotype in recurrent pregnancy loss

Fig. 4

a Tconv and (b) Treg were enumerated by flow cytometry in nulliparous and parous controls (NulC (n = 6) and ParC (n = 8)—black bars) and primary and secondary RPL (PRPL (n = 12) and SRPL (n = 7)—red bars), (c) the Treg proportion of the CD4 Tconv population versus the number of miscarriage in PRPL (triangles) and SRPL (crosses). Treg gene expression signatures were compared analysing uniquely expressed and shared genes, displayed as VENN diagrams, and top 10 differentially upregulated and downregulated expressed genes, displayed as heatmaps, between (d, e) NulC and PRPL Treg or (f, g) ParC and SRPL, respectively. Flow cytometric analysis of cell surface (h, i) S1PR1 (n = 3 each group) and (j) TIGIT (n = 16, 14, 4, 6 in order) between RPL and control patients showing mean fluorescent intensity (mfi) (h and j) or the % positive Treg cells (i) detected by flow cytometry. Bars = mean value.

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