Fig. 2: Dendritic cells from tolerized mice suppress Th2 phenotype in co-cultured naïve CD4+ T cells. | Mucosal Immunology

Fig. 2: Dendritic cells from tolerized mice suppress Th2 phenotype in co-cultured naïve CD4+ T cells.

From: Early life exposure to house dust mite allergen prevents experimental allergic asthma requiring mitochondrial H2O2

Fig. 2

24 h after the last HDM sensitization regimen, DCs purified from lungs of WT mice sensitized to high-dose HDM (5.0 µg) or low-dose HDM (0.5 µg) were co-cultured with naive CD4+ T cells from OT-II mice in vitro for 5 days in the presence of OVA peptide. DCs in each group were pooled from 3 to 5 mice. a, c, e Representative flow plots of the percentage of Th2 cells (GATA-3+IL-13+) (gated on live CD4+ CD25+ cells), total IL-10+ population (gated on live CD4+ cells), FoxP3 IL-10+, and FoxP3+ IL-10+ populations (gated on live CD4+ cells) in the lungs. b, d, f Quantifications of the percentages of cell types shown in (a, c, e) in each mouse. g ELISA measurements of the indicated cytokines present in culture supernatants after 5 days of co-culture. Data shown are median ± IQR combined from three independent experiments that includes technical replicates in each group. Each data point represents cytokine level in a single well. Two-tailed Mann–Whitney U test (b, d, f, g). *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; nsP > 0.05.

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