Fig. 3: NECA alleviates ILC2-mediated airway inflammation.

a WT mice were challenged with PBS or papain i.n for 5 consecutive days and simultaneously treated with NECA or DMSO every day intraperitoneally, mice were sacrificed 24 h after last challenge. b EOS levels in BALF were analyzed by flow cytometry. c The amount of IL-5 and IL-13 in BALF was examined by ELISA. d H&E staining of lung tissues (bars, 100 µm). e Flow cytometric analysis the frequencies and absolute number of ILC2s in lung. f, g Frequencies of Ki67⁺ ILC2(f) and IL-5⁺IL-13⁺ ILC2(g) in lung. Data are representative of two independent experiments (n = 4 for PBS groups, n = 6 for papain groups). Error bars show mean ± SEM; *P < 0.05; **P < 0.01; ***P < 0.001 by unpaired Student’s t test.