Fig. 4: Blocking the synthesis of adenosine aggravates lung inflammation.

WT mice were challenged with PBS or papain i.n for 5 consecutive days and simultaneously treated with APCP (20 mg/kg/day) or control vehicle every day intraperitoneally, mice were sacrificed 24 h after last challenge. The amount of adenosine in lung tissue (a) or BALF (b). c EOS plots and absolute number in BALF were analyzed by flow cytometry. d The amount of IL-5 and IL-13 in BALF was examined by ELISA. e H&E staining of lung tissues (bars, 100 µm). f Flow cytometric analysis of lung ILC2. Frequencies of Ki67⁺ ILC2 (g) and IL-5⁺IL-13⁺ ILC2 (h) in lung. Data are representative of two independent experiments (n = 4 for PBS groups, n = 6 for papain groups). Error bars show mean ± SEM; **P < 0.01; ***P < 0.001 by unpaired Student’s t test.