Fig. 5: Adenosine suppresses ILC2 function though A2A receptor.

a–c Lung ILC2 were cultured in the presence of IL-2 (20 ng/ml), IL-7 (20 ng/ml) and IL-33 (20 ng/ml) for 3 days and treated with DMSO, NECA (1 μM) + DMSO, NECA (1 μM) + SCH58261(1 μM) or NECA + MRS1754 (2 μM). Flow cytometric analysis of IL-5⁺IL-13⁺ (a) and Ki67⁺ (b) cultured ILC2. c IL-5 and IL-13 amount in supernatant were determined by ELISA. d–f Lung ILC2 were cultured in the presence of IL-2, IL-7 and IL-33 for 3 days and treated with DMSO, NECA (1 μM), CGS 21680 (10 μM) or BAY 60-6583 (10 μM). Flow cytometric analysis of IL-5⁺IL-13⁺ (d) and Ki67⁺ (e) cultured ILC2. f IL-5 and IL-13 amount in supernatant were determined by ELISA. Data are representative of two independent experiments. Error bars show mean ± SEM; *P < 0.05; **P < 0.01; ***P < 0.001 by one-way ANOVA with Bonferroni post-test.