Fig. 6: A2A^−/− mice exhibits aggravated lung inflammation.

WT and A2A^−/− mice were challenged with PBS or papain i.n for 5 consecutive days and sacrificed 24 h after last challenge. a EOS levels in BALF were analyzed by flow cytometry. b The amount of IL-5 and IL-13 in BALF was examined by ELISA. c H&E staining of lung tissues (bars, 100 µm). d Flow cytometric analysis of lung ILC2. Frequencies of Ki67⁺ ILC2 (e) and IL-5⁺IL-13⁺ ILC2 (f) in lung. Data are representative of two independent experiments (n = 4 for PBS groups, n = 6 for papain). Error bars show mean ± SEM; *P < 0.05; **P < 0.01; ***P < 0.001 by unpaired Student’s t test.