Fig. 1: B cells of AR patients express lower ASCT2 levels.
From: Glutaminolysis is required in maintaining immune regulatory functions in B cells
PBMCs were isolated from blood samples collected from AR patients (n = 50) and HC patients (n = 50) and analyzed by FACS. a The FSC/SSC plots. b Gated FACS plots show CD19+ B cell frequency. c Boxplots show summarized CD19+ B cell counts. d, e Gated FACS plots show IL-10+ CD19+ B cells (B10 cells); boxplots show summarized B10 cell counts. f, g Gated FACS histograms show ASCT2+ IL-10− B cells and B10 cells; boxplots show MFI of ASCT2 in IL-10− B cells and B10 cells against the total MFI. h, i Gated FACS histograms show Glut1+ IL-10− B cells and B10 cells; boxplots show MFI of Glut1 in IL-10− B cells and B10 cells. ***p < 0.001 (Mann Whitney-U test), compared with the HC group. Each dot in boxplots presents data obtained from one sample (samples were tested in triplicate). The FACS plots are from one experiment, that represent 50 independent tests. The group labels of g, i are the same as that in FACS histograms on the left side.
