Fig. 3: Assessment of the role of glutamine catabolism in B10 cell induction.
From: Glutaminolysis is required in maintaining immune regulatory functions in B cells
B cells isolated from HC subjects were cultured in the conditions denoted above each FACS plot for 3 days. CD40L: 1 µg/ml. CpG: 10 µg/ml. BPTES: 10 µM. DMOG: 1 mM. glutamine-d: glutamine-deficient medium. Glucose-d: Glucose-deficient medium. a Gated FACS plots show CD35+ IL-10+ B cell frequency. b Boxplots show B10 cell counts from ten independent experiments. Boxplots show IL-10 mRNA in B cells (c) and IL-10 protein in culture supernatant (d). ***p < 0.001 (ANOVA followed by the Dunnett’s test), compared with group a. The group labels of b are the same as those in a. Each dot in boxplots presents data obtained from one sample. The FACS plots of a are from one experiment that represent six independent experiments. Exposure to BPETS solvent, DMSO (0.5 μl/ml), did not alter the IL-10 expression in B cells (data not shown). Cell viability at the end of culture was greater than 99%.
