Fig. 4: mTORc1 mediates the effects of glutamine on inducing IL-10 in B cells.
From: Glutaminolysis is required in maintaining immune regulatory functions in B cells
a–d B cells were isolated from blood samples collected from HC subjects and AR patients, and exposed to reagents listed below the immunoblots of a. Glutamine: glutamine in the culture medium. Glutamine-d: glutamine-deficient culture medium. Rapa: Rapamycin in the culture (10 nM). CD40L: 1 µg/ml. CpG: 10 µg/ml. The immunoblots show phosphor mTORc1 (p-mTORc1) (a) and phosphor S6 (pS6) (c) in B cells. The scatter plots show integrated density of p-mTORc1 blots (b) and pS6 blots (d). e The scatter dot plots show the IL-10 levels in culture supernatant. The boxplots show positive correlation between the IL-10 levels in supernatant and the pmTORc1 levels (f) and the pS6 levels (g) in B cells. ***p < 0.001 (ANOVA followed by the Dunnett’s post hoc test), compared with the saline group. The data represent ten independent experiments.
