Fig. 6: Impaired IL-10 expression in Crohn’s disease patients is associated with reduced Blimp-1/c-Maf expression. | Mucosal Immunology

Fig. 6: Impaired IL-10 expression in Crohn’s disease patients is associated with reduced Blimp-1/c-Maf expression.

From: A Notch/STAT3-driven Blimp-1/c-Maf-dependent molecular switch induces IL-10 expression in human CD4+ T cells and is defective in Crohn´s disease patients

Fig. 6

a Effector memory CD4+ T cells were isolated from blood of healthy donors (HD) or Crohn’s disease (CD) patients. Afterwards, cells were cultured and co-stimulated w/ or w/o DLL4/Cyt (Cyt = IFN-α/IL-6/IL-21) for 120 h. Quantification of Blimp-1 and c-Maf MFI among effector memory CD4+ T cells (left) and representative histograms (right). b Quantification of Blimp-1 and c-Maf MFI among Th1 (IFN-γ+), Th17 (IL-17A+) and Th1/17 (IFN-γ+IL-17A+) cells from HD and CD patients co-stimulated w/ or w/o DLL4/Cyt (Cyt = IFN-α/IL-6/IL-21). Each dot represents one donor (nHD=13, nCD = 9). Two-tailed Mann–Whitney and unpaired t tests were performed (* P < 0.05; ** P < 0.01; *** P < 0.001) to assess significance. Data are cumulative from four independent experiments.

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