Fig. 6: Highly susceptible C57BL/6 mice display more extensive local Th2/1 hybrid accumulation next to more prominent Th1 cells.

a Frequencies of T-bet expressing cells in CD4+ cells in mLN, spleen and small intestine of naïve BALB/c and C57BL/6 mice at the age of 1.5 months. Data from three independent experiments (n = 6–7 mice/group) are pooled. b Fecal egg counts of H. polygyrus infected BALB/c and C57BL/6 mice over the course of five weeks. Data derive from one out of two experiments with similar outcome (n = 5 mice/line) and are reported as mean ± SD. c Adult worm counts at day 35 post infection. Data from three independent experiments (n = 10–11 mice/group) are pooled. d T-bet and GATA-3 expression in CD4+ Foxp3− cells in spleen and small intestine at d35 post infection. Statistics are provided in Supplementary Fig. S3. e Stacked bars depicting small intestinal IFN-γ responses by CD4−, Th1 and Th2/1 cells shown from one dataset representative for 2-3 experiments as mean ± SD. n = 3–6 per infection time point. Naïve controls (n = 5 and 8) are pooled from four individual experiments. Significant differences between the mouse lines at a given time point concerning the total and cell type-specific IFN-γ signals are indicated by (§) and (*), respectively. f Frequencies of Th2/1, Th2 and Th1 cells in CD4+ FoxP3− cells in small intestine. Data from two (C57BL/6) or three (BALB/c) experiments are pooled (n BALB/c naïve = 7, C57BL/6 naïve = 4; BALB/c infected = 10, C57BL/6 infected = 6 mice) and reported as mean + SD. g Quantification of L4 migrated from small intestinal tissue explants within 4 h, expressed as percentage of the total larvae present in tissue. Data from two experiments each performed with four BALB/c and three C57BL/6 mice per group are pooled. h Ratio of Th2 to Th2/1 cells at day 14 in mLN, spleen and small intestine. Data from 2 to 3 individual experiments each performed with 3–5 mice/group are reported. i Mean egg production of individual female worms within 24 h of culture. Data from 2 independent experiments each performed with n = 3 mice/group are pooled. Bars in a, c, g, h and i report the mean, circles represent individual mice. Statistical analysis was performed using unpaired t test or Mann–Whitney test in a–c, e and g, and one-way ANOVA with Tukey’s multiple comparisons test or Kruskal–Wallis test with Dunn’s multiple comparisons test in f, h and i. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001.