Fig. 1: Hoil1−/− mice exhibit type 2 inflammation in the distal ileum.
From: HOIL1 regulates group 2 innate lymphoid cell numbers and type 2 inflammation in the small intestine
a Representative images of H&E stained sections of ileum from Hoil1+/+ and Hoil1−/− mice. Scale bars represent 50 μm (left panel) and 20 μm (right panels). b Representative images of PAS/Alcian Blue stained sections. c Goblet cells per villus in Hoil1+/+ and Hoil1−/− ileum. d–h Relative Il4, Il5, Il13 (d), Tnf, Ifng (f), Il25, Il33, Il18 and Tslp (g) mRNA levels, and IL-4, IL-5 (e), IL-25, IL-33 and TSLP (h) protein levels in ileum of Hoil1+/+ and Hoil1−/− mice. I Il18, Il25, Il33 and Tslp mRNA levels in Hoil1+/+ and Hoil1−/− IEC and LP fractions relative to Hoil1+/+ IEC median. j DCLK1 (red) and DAPI (blue) stained sections of ileum from Hoil1+/+ (left) and Hoil1−/− (right) mice (scale = 50 μm). k Enumeration of tuft cells in villi and crypts of Hoil1+/+ and Hoil1−/− ileum. Each symbol represents a sample from an individual mouse and colored bars represent the median. mRNA levels are expressed as relative to the median level for Hoil1+/+. Histological enumerations and measurements represent the mean from >10 villi per mouse. All mice were aged between 6 and 9 weeks. H&E Hematoxylin and Eosin. IEC Intestinal epithelial cell fraction, LP Lamina propria fraction. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.001 by Mann-Whitney (c–h, k) or ordinary 2-way ANOVA (i).
