Fig. 3: Elevated type 2 inflammatory cytokine expression in Hoil1−/− ileum is not dependent on feed-forward signaling in IECs.
From: HOIL1 regulates group 2 innate lymphoid cell numbers and type 2 inflammation in the small intestine
a H&E, b PAS/Alcian Blue, c DCLK1 and DAPI, and d lysozyme and DAPI stained sections of ileum from Hoil1+/+Il4raf/f, Hoil1+/+Il4raΔIEC, Hoil1−/−Il4raf/f and Hoil1−/−Il4raΔIEC mice (scale = 50 μm). e Goblet (left panel) and tuft (right panel) cells per villus in Hoil1+/+Il4raf/f, Hoil1+/+Il4raΔIEC, Hoil1−/−Il4raf/f and Hoil1−/−Il4raΔIEC mice. f, g Relative Il25, Il33, Tslp (f), Il4, Il5 and Il13 (g) mRNA levels in ileum from Hoil1+/+Il4raf/f, Hoil1+/+Il4raΔIEC, Hoil1−/−Il4raf/f and Hoil1−/−Il4raΔIEC mice. h, i IL-25, IL-33, TSLP (h), IL-4, IL-5 and IL-13 (i) protein levels in distal ileum from Hoil1+/+Il4raf/f, Hoil1+/+Il4raΔIEC, Hoil1−/−Il4raf/f and Hoil1−/−Il4raΔIEC mice. j–l Relative Il13, Il5 and Il4 mRNA levels in jejunum (j), MLN (k) and distal colon (l) from Hoil1+/+Il4raf/f, Hoil1+/+Il4raΔIEC, Hoil1−/−Il4raf/f and Hoil1−/−Il4raΔIEC mice. Each symbol represents a sample from an individual mouse and colored bars represent the median. mRNA levels are expressed as relative to the median for Hoil1+/+Il4raf/f. Histological enumerations and measurements represent the mean from >10 villi per mouse. All mice were aged between 6 and 9 weeks. H&E Hematoxylin and Eosin. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.001 by 2-way ANOVA with Tukey’s multiple comparisons test (E-K) or Mann Whitney test (l).
