Fig. 4: T cells are not required to drive type 2 inflammation in Hoil1−/− ileum.
From: HOIL1 regulates group 2 innate lymphoid cell numbers and type 2 inflammation in the small intestine
a Il4, Il5 and Il13 mRNA levels in Hoil1+/+ and Hoil1−/− IEC and LP cell fractions relative to Hoil1+/+ IEC. b Representative flow plots gated on live, CD45+ LP cells from Hoil1+/+ and Hoil1−/− ileum showing the gating strategy and intracellular IL-13 expression in CD3+ and in CD11b- CD3- CD19- cell populations. c Quantification of IL-13+ cells (left panel) and percentage (of total CD45+ cells, right panel) for the indicated cell populations from Hoil1+/+ and Hoil1−/− ileum. d, e H&E (d) and DCLK1 and DAPI (e) stained sections of ileum from Hoil1+/+Rag1−/− and Hoil1−/−Rag1−/− mice (scale = 50 μm). f Enumeration of goblet cells (left panel) and tuft cells (right panel) per villus in ileum from Hoil1+/+Rag1−/− and Hoil1−/−Rag1−/− mice. g, h Il4, Il5, Il13 (g), Il25 and Il33 (h), mRNA levels in Hoil1+/+Rag1−/− and Hoil1−/−Rag1−/− distal ileum relative to Hoil1+/+Rag1−/−. Each symbol represents a sample from an individual mouse and bars represent the median. All mice were aged between 6–9 weeks. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001 by 2-way ANOVA with Tukey’s multiple comparisons test (a) or Mann-Whitney test (c, f–h).
