Fig. 5: Expression of HOIL1 in non-hematopoietic cells is required to suppress type 2 inflammation in the ileum.
From: HOIL1 regulates group 2 innate lymphoid cell numbers and type 2 inflammation in the small intestine
a Rbck1 mRNA levels (Hoil1, exons 3–4) in ileum from bone marrow chimeric mice relative to WT + WT. WT + WT: Hoil1+/+ mice with Hoil1+/+ bone marrow; WT + KO: Hoil1+/+ mice with Hoil1−/− bone marrow; KO + WT: Hoil1−/− mice with Hoil1+/+ bone marrow; and KO + KO: Hoil1−/− mice with Hoil1−/− bone marrow. b Percentage of WT (Rbck1 intron 7, left panel) or KO (neomycin-resistance cassette, right panel) gDNA in blood from bone marrow chimeric mice relative to WT + WT or KO + KO controls. c Il13 mRNA levels in ileum from bone marrow chimeric mice relative to WT + WT. d Representative H&E stained sections of ileum from bone marrow chimeric mice (scale = 50 μm). e Enumeration of goblet cells per villus in ileum from bone marrow chimeric mice. Each symbol represents a sample from an individual mouse and colored bars represent the median. Histological enumerations and measurements represent the mean from >10 villi per mouse. Chimeric mice were analyzed 16 weeks after reconstitution. H&E Hematoxylin and Eosin. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.001 by Brown-Forythe and Welch one-way ANOVA with Dunnett’s T3 multiple comparisons test (a–c), or ordinary one-way ANOVA with Tukey’s multiple comparisons test (e).
