Fig. 7: HOIL1 regulates ILC2 numbers in the small intestine.
From: HOIL1 regulates group 2 innate lymphoid cell numbers and type 2 inflammation in the small intestine
a Counts per million reads (CPM) for the indicated genes identified as differentially expressed in CD45+ cells from Hoil1−/−Il4raf/f and Hoil1−/−Il4raΔIEC ileum relative to Hoil1+/+Il4raf/f and Hoil1+/+Il4raΔIEC by RNA-Seq analysis. q < 0.05 for both comparisons. b Heat map/dot plot representation of the indicated genes in cell types identified in LP from untreated and OVA-treated mice by scRNAseq29. c Heat map/dot plot representation of Rbck1 expression in intestinal epithelial cell subsets identified by scRNAseq30. d, e mRNA levels for the indicated genes in whole distal ileum (d) or MLN (e) measured by qRT-PCR and expressed as relative to Hoil1+/+Il4raf/f. f Representative flow plots gated on live, CD45+, Lin- (CD3, CD4, CD5, CD11b, CD11c, CD19, NK1.1) cells showing expression of CD90.2 and KLRG1. g Quantification of panel F for Lin- CD90.2- KLRG1+ and Lin- CD90.2+ KLRG1+ cells (percentage of total CD45+ cells). Each symbol represents a sample from an individual mouse and colored bars represent the median. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001 by 2-way ANOVA with Tukey’s multiple comparisons test.
