Fig. 3: Human inner foreskin langerin-expressing cells are productively infected with HSV-1 and HSV-2. | Mucosal Immunology

Fig. 3: Human inner foreskin langerin-expressing cells are productively infected with HSV-1 and HSV-2.

From: CGRP inhibits human Langerhans cells infection with HSV by differentially modulating specific HSV-1 and HSV-2 entry mechanisms

Fig. 3

Inner foreskin epidermal cell suspensions were pulsed for 24 h with primary isolates of HSV-1 (isolate 3) or HSV-2 (isolate 8) at MOI = 10.0, stained at 48 h pi for surface langerin and intracellular HSV-1/2-gD, and examined by flow cytometry. a Representative flow cytometry dot-plots of suspensions infected with HSV-1 or HSV-2 vs. no HSV control, with numbers representing percentages of langerinhighHSV-1/2− (upper left regions), langerinhighHSV-1/2+ (upper right regions), langerinlowHSV-1/2− (middle left regions) and langerinlowHSV-1/2+ (middle right regions) cells. b Matched infection percentages of inner foreskin langerinhigh (dark grey) vs. langerinlow (light grey) cells, infected with either HSV-1 (dots) or HSV-2 (triangles). For HSV-2, **p = 0.0023; paired two-tailed Student’s t-test.

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