Fig. 4: LPS exposure persistently alters the metabolic state and responsiveness of AMs.
From: Trained immunity of alveolar macrophages requires metabolic rewiring and type 1 interferon signaling
a Experimental setup for Seahorse analyses of AMs on day six after in vivo training with LPS/saline. b OCR and ECAR of trained and control AMs, measured at baseline and after sequential treatment with oligomycin (Oligo), FCCP and rotenone/antimycin A (R/A). c Quantification of baseline OCR (basal respiration), ATP production rate and baseline ECAR. d Experimental setup for Seahorse analyses of AMs upon ex vivo HISP or medium challenge (16 h) on day six after training. e OCR and ECAR of trained and control AMs, 16 h after HISP stimulation, measured at baseline and after sequential treatment with indicated drugs. f Quantification of basal respiration, ATP production rate and baseline ECAR 16 h after HISP challenge. g Quantification of baseline OCR and ECAR 16 h after stimulation with HISP in medium versus medium only. Graphs show means + SEM of 10-11 technical replicates from pooled biological replicates (n = 5-8). Data are representative of two independent experiments. Statistical analysis: student’s t-test. ns, not significant. ***p ≤ 0.001, ****p ≤ 0.0001; OCR, oxygen consumption rate; ECAR, extracellular acidification rate; FCCP, carbonyl cyanide-4-(trifluoromethoxy)phenylhydrazone.
