Fig. 4: Influence of TRPV1 blockade on corneal wound healing and inflammation after epithelial abrasion.

a Observation of corneal wound closure in vehicle-treated mice and AMG-517 treated mice. Images on the left show the staining of corneal wounds at each time point after epithelial abrasion with fluorescein sodium. The graph on the right shows the dynamic changes in the ratio of wounded area within the whole corneal area of vehicle-treated mice and AMG-517 treated mice (n = 6 mice per group). b Comparison of the number of dividing epithelial cells at each time point (left) and at all time points (right) after epithelial abrasion in vehicle-treated mice and AMG-517 treated mice (n = 6 mice per group at each time point). c Analysis of the difference in the number of neutrophils (left) and γδ T cells (right) in the cornea of vehicle-treated mice and AMG-517 treated mice at each time point after epithelial abrasion using immunostaining of corneal whole-mount (n = 6 mice per group at each time point). d Comparison of the number of neutrophils (left, n = 3 independent experiments, 5 mice per experiment in each group) and γδ T cells (right, n = 3 independent experiments, 5 mice per experiment in each group) in the cornea of vehicle-treated mice and AMG-517 treated mice at 18 h after epithelial abrasion using flow cytometric analysis. Data are presented as mean ± SD. *P < 0.05, **P < 0.01, and ***P < 0.001.