Fig. 2: Forced CCR9 expression does not interfere with CD4SP thymocyte development.
a Identification of pre- (CCR7–TCRβlo) and post-selection (CCR7+TCRβhi) thymocytes of WT and CCR9Tg mice. Total thymocyte numbers are listed on the top. Bar graph shows post-selection thymocyte frequencies as a summary of 4 independent experiments with a total of 4 WT and 4 CCR9Tg mice. b Graphs show the delta Median Fluorescence Intensity (ΔMFI) and geometric Mean Fluorescence Intensity (ΔgMFI) of CCR9 on pre- and post-selection thymocytes of WT and CCR9Tg mice, respectively. Data are from 4 independent experiments with a total of 4 WT and 4 CCR9Tg mice. c Contour plots show the SM, M1, and M2 distribution of post-selection thymocytes in WT and CCR9Tg mice. Data are from 4 independent experiments with a total of 4 WT and 4 CCR9Tg mice. Statistical significance was determined by two-way ANOVA with Sidak’s multiple comparisons test. d Surface abundance of CCR9 was determined on SM, M1, or M2 CD4SP thymocytes of WT and CCR9Tg mice. Histograms (top) are representative and bar graphs (bottom) show summary from 4 independent experiments with a total of 4 WT and 4 CCR9Tg mice. e CD4 versus CD8 profiles of total (top) and TCRβhi-gated (middle) mature thymocytes (bottom) of WT and CCR9Tg mice. Data are representative of 5 independent experiments with a total of 5 WT and 5 CCR9Tg mice. f Frequencies and numbers of TCRβhi (top) and mature CD4SP thymocytes (bottom) from WT and CCR9Tg mice. Data show summary of 5 independent experiments with a total of 5 WT and 5 CCR9Tg mice. Data are shown as mean ± SEM. **P < 0.01, ***P < 0.001, n.s., not significant.
