Fig. 5: Forced expression of CCR9 is detrimental for the differentiation of DP IEL T cells.
a Frequencies and numbers of DP IEL T cells in the SI epithelium of WT and CCR9Tg mice. Contour plots are representative, and bar graphs show summary of 7 independent experiments with a total of 10 WT and 7 CCR9Tg mice. b DP IEL frequencies among CD4+CD8β– T cells of WT and CCR9Tg mice. Contour plots are representative, and bar graphs show summary of 5 independent experiments with a total of 6 WT and 5 CCR9Tg mice. c Frequencies of Foxp3-GFP+ Tregs in CD4SP and DP SI IEL T cells of Foxp3-GFP reporter and Foxp3-GFP-CCR9Tg mice. Contour plots are representative, and bar graph shows summary of 3 independent experiments with a total of 4 Foxp3-GFP and 3 Foxp3-GFP-CCR9Tg mice. Statistical significance was determined by two-way ANOVA with Sidak’s multiple comparisons test. d DP IEL frequencies among CD4+CD8β– T cells of WT and CCR9KO mice. Contour plots are representative (left), and bar graphs (right) show summary of 4 independent experiments with a total of 6 WT and 6 CCR9KO mice. Data are shown as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, n.s., not significant.
