Fig. 6: Tissue-specific mapping of CCR9-mediated differentiation of DP IEL T cells.

a Frequencies of DP IEL T cells in WT mice were assessed in the proximal (duodenum/jejunum) and distal (ileum) parts of the SI epithelium. Contour plots are representative (left), and bar graph (right) shows summary of 2 independent experiments with a total of 4 WT mice. Statistical significance was determined by paired two-tailed Student’s t-test. b Chemokine expression in SI epithelial cells (ECs) was assessed using public RNA-seq datasets and visualized as violin plots for individual chemokines. c Heatmap of chemokine expression in different parts of SI ECs. Public RNA-seq data of SI ECs from the duodenum, jejunum, and ileum were analyzed, and the site-specific chemokine expression was assembled as heatmap. d RT-qPCR analyses of CCL25 mRNA expression normalized to β-actin mRNA in the indicated gut tissues of WT mice. Data show summary of 2 independent experiments. Statistical significance was assessed by one-way ANOVA with Tukey’s multiple comparisons test. e Frequencies of DP IELs in different parts of the SI epithelium of WT and CCR9^Tg mice. Contour plots are representative (left), and bar graph (right) shows summary of 4 independent experiments with a total of 5 WT and 4 CCR9^Tg mice. Statistical significance was determined by two-way ANOVA with Sidak’s multiple comparisons test. f Frequencies of DP IELs in the indicated parts of the SI epithelium of WT and CCR9^KO mice. Contour plots are representative (left), and bar graph (right) shows summary of 4 independent experiments with a total of 5 WT and 4 CCR9^KO mice. Statistical significance was determined by two-way ANOVA with Sidak’s multiple comparisons test. Data are shown as mean ± SEM. **P < 0.01, ***P < 0.001, n.s., not significant.