Fig. 1: Human regulatory T cells in the vaginal mucosa display increased activation potential compared to circulating Tregs. | Mucosal Immunology

Fig. 1: Human regulatory T cells in the vaginal mucosa display increased activation potential compared to circulating Tregs.

From: Mucosal viral infection induces a regulatory T cell activation phenotype distinct from tissue residency in mouse and human tissues

Fig. 1

a Peripheral blood mononuclear cell (PBMC) and paired mucosal biopsies from the vaginal tract (VT) were obtained from 14 healthy adult women with no genital tract infections. b Tregs were identified by flow cytometry as CD4+ CD127− CD25+ FoxP3+ cells. c Donor-matched frequency of Tregs out of total CD4+ T cells in PBMC and VT. d Representative CD69 and CD103 staining, gated on Tregs (left), and CD69+ and CD103+ fractions of CD8, CD4 Tconv, and Treg populations from PBMC and VT, as a percentage of parent population (right). e CD69+ CD103− (left) and CD69+ CD103+ (right) fractions of CD8, CD4 Tconv, and Tregs from VT. f Representative Treg activation marker staining on PBMC (red) and VT (blue), gated on Tregs (top). Donor-matched frequencies of activation markers in PBMC and VT, gated on Tregs (bottom). n = 14, Significance defined by paired t test; p < 0.05.

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