Fig. 2: Vaginal tissue Tregs are highly activated compared to lymphoid tissue Tregs in healthy mice. | Mucosal Immunology

Fig. 2: Vaginal tissue Tregs are highly activated compared to lymphoid tissue Tregs in healthy mice.

From: Mucosal viral infection induces a regulatory T cell activation phenotype distinct from tissue residency in mouse and human tissues

Fig. 2

C57BL/6 J mice were administered Depo provera s.c. in the neck ruff, and VT and vaginal-draining lymph nodes (dLN) were harvested 5–7 days later. a Tregs were identified as CD4+ FoxP3+ by flow cytometry. b Representative staining for CTLA-4, GITR, ICOS, and CD69 on FoxP3+ Tregs (left; VT plot is the concatenated image of 5 individual VT tissues), quantified as a percentage of total FoxP3+ Tregs (right). n = 5; data representative of 3 replicate experiments. Significance defined by paired t test; p < 0.05.

Back to article page