Fig. 3: Vaginal HSV-2 infection increases the accumulation of highly activated Tregs at the site of infection and drives increased expression of select activation markers consistent with a tissue signature. | Mucosal Immunology

Fig. 3: Vaginal HSV-2 infection increases the accumulation of highly activated Tregs at the site of infection and drives increased expression of select activation markers consistent with a tissue signature.

From: Mucosal viral infection induces a regulatory T cell activation phenotype distinct from tissue residency in mouse and human tissues

Fig. 3

C57BL/6 J mice were administered Depo provera s.c. in the neck ruff, and infected 5-7 days later with intravaginal (ivag.) HSV-2. On days 0 (uninfected [uninf.]), 3, and 7 post-infection, VT and dLN were harvested for flow cytometry analysis. a Tregs were identified as CD4+FoxP3+ by flow cytometry. Representative Treg staining in dLN (grey) and VT (blue) on day 3 post-infection (left). Treg frequency was quantified as a percentage of total CD4+ T cells and absolute number of Tregs on days 0, 3, and 7 (right). b Representative staining for CTLA-4, GITR, ICOS, and CD69 on FoxP3+ Tregs on day 7 post-infection (left), quantified as a percentage of total FoxP3+ Tregs and absolute number on days 0, 3, and 7 (right). n = 5; data representative of 3 replicate experiments. Significance defined by paired t test or Kruskal–Wallis test followed by Dunn’s multiple comparison test; p < 0.05.

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