Fig. 4: Vaginal Tregs are transcriptionally distinct from dLN Tregs and enriched for visceral adipose tissue Treg gene signature.
FoxP3GFP mice were administered Depo provera s.c. in the neck ruff and infected ivag 5–7 days later with WT HSV-2. On day 7 post-infection, VT and dLN were harvested and prepared for fluorescence-activated cell sorting (FACS) to isolate CD4+ FoxP3+ Tregs. Bulk RNA-sequencing was performed on Tregs from dLN and VT. a Significantly differentially expressed genes (DEG) in dLN and VT Tregs were defined by a log2FC greater than 1 and a false discovery rate (FDR) < 0.05 and visualized in a heatmap. n = 4. b Gene set enrichment analysis (GSEA) of dLN and VT Tregs day 7 post-infection compared to a previously published gene signature for visceral adipose tissue Tregs9. n = 4; DEG defined by FDR < 0.05.
