Fig. 6: Inflammatory cytokines induce Granzyme B expression in Tregs. | Mucosal Immunology

Fig. 6: Inflammatory cytokines induce Granzyme B expression in Tregs.

From: Mucosal viral infection induces a regulatory T cell activation phenotype distinct from tissue residency in mouse and human tissues

Fig. 6

Bulk dLN cells from a healthy C57BL/6 J mouse were cultured in various cytokine conditions as indicated. a After 24 h, CD4+ FoxP3+ Tregs (top), CD4+ FoxP3− CD4Tconv (middle), and CD8+ T cells (bottom) were stained for GzmB expression by flow cytometry. Example staining (left) Tregs, CD4 Tconv, or CD8 expressing GzmB as a percentage of total FoxP3+, CD4+ FoxP3−, or CD8+, respectively (right). b Bulk dLN cells from a healthy mouse were enriched for T cells using magnetic separation and cultured in various cytokine conditions. After 24 h, CD4+ FoxP3+ Tregs (top), CD4+ FoxP3− CD4Tconv (middle), and CD8+ T cells (bottom) were stained for GzmB expression by flow cytometry. Example staining (left) and Tregs, CD4 Tconv, or CD8 expressing GzmB as a percentage of total FoxP3+, CD4− FoxP3+, or CD8+, respectively (right). Data representative of two replicate experiments. Significance defined by one-way ANOVA followed by Tukey’s test for multiple comparisons; p < 0.05.

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