Fig. 7: IL-2 depletion in vivo decreases vaginal Treg expression of GzmB. | Mucosal Immunology

Fig. 7: IL-2 depletion in vivo decreases vaginal Treg expression of GzmB.

From: Mucosal viral infection induces a regulatory T cell activation phenotype distinct from tissue residency in mouse and human tissues

Fig. 7

C57BL/6 J mice were administered Depo provera s.c. in the neck ruff and infected ivag 5–7 days later with HSV-2. On day 3 p.i., mice were administered both intravenous (i.v.) and intravaginal (ivag.) anti-IL-2 antibody (aIL-2) or PBS control, and treated with ivag. aIL-2 or PBS on days 4–6. On day 7 or 8 p.i., dLN and VT were harvested for flow cytometry analysis. Tregs and CD8 T cells were identified as CD4+ FoxP3+ and CD8+, respectively. Representative granzyme B (GzmB) ex vivo staining in dLN (grey) and VT (blue) in a Tregs and b CD8 on day 7 post infection (left), quantified as a percentage of total Treg or CD8+ (right). n = 4 HSV-2 + aIL-2, n = 3 HSV-2 + PBS, n = 2 uninfected + aIL-2, n = 1 uninfected + PBS. Data representative of 2 replicate experiments. Significance defined by one-way ANOVA followed by Tukey’s test for multiple comparisions; p < 0.05.

Back to article page