Fig. 2: Age did not distinguish epithelial susceptibility and immune responses to SARS-CoV-2, which were minimal compared to H1N1.
From: Distinct airway epithelial immune responses after infection with SARS-CoV-2 compared to H1N1
a, b Representative images of immunofluorescence staining for SARS-CoV-2 nucleocapsid protein (NP) (magenta), MUC5AC (yellow) and KRT5 (cyan) in SARS-CoV-2-infected cells at 3 (A) or 5 (B) days post-infection (dpi). Size of scale bar: 50 µm. c Viral titres in apical wash of epithelial cells infected with SARS-CoV-2 or H1N1 as determined by plaque assays. Line graph in (A) shows median + confidence interval. d Principal component analysis of SARS-CoV-2-infected cells based on 121 variables (viral titres, expression of 28 genes and concentration of 2 proteins across up to five time points). Each data point represents one donor. e Principal component analysis of epithelial cells based on 98 variables (expression of 28 genes and concentration of 2 proteins across up to five time points). Each data point represents one treatment of one donor.
