Table 3 Antibodies used for immunofluorescence staining.
From: Distinct airway epithelial immune responses after infection with SARS-CoV-2 compared to H1N1
Primary antibodies | |||
|---|---|---|---|
Antibody (clone) | Conjugate | Supplier (catalogue #) | Concentration |
ACE-2 | – | R&D Systems (AF933) | 4.0 µg/ml |
Mucin 5AC (EPR16904) | AF555 | abcam (ab218714) | 5.0 µg/ml |
Cytokeratin 5 (EP1601Y) | AF647 | abcam (ab193895) | 2.5 µg/ml |
Goat IgG, polyclonal (isotype) | – | abcam (ab37373) | 4.0 µg/ml |
SARS Nucleocapsid protein | – | Novus Bio (NB100-56576) | 10.0 µg/ml |
Alpha Tubulin (6-11B-1) | – | abcam (ab24610) | 10.0 µg/ml |
Rabbit IgG, Control Antibody | – | Vector Labs (I-1000-5) | 10.0 µg/ml |
Secondary antibodies | |||
|---|---|---|---|
Antibody | Conjugate | Supplier (catalogue #) | Concentration |
Donkey anti-mouse IgG | AF488 | Invitrogen (A-21202) | 10.0 µg/ml |
Donkey anti-rabbit IgG | AF594 | Invitrogen (A-21207) | 10.0 µg/ml |
Donkey anti-goat IgG | AF488 | Invitrogen (A-11055) | 10.0 µg/ml |
