Fig. 2: Lack of IκBζ in IECs results in marked expansion of SFB in the small intestine. | Mucosal Immunology

Fig. 2: Lack of IκBζ in IECs results in marked expansion of SFB in the small intestine.

From: IκBζ controls IL-17-triggered gene expression program in intestinal epithelial cells that restricts colonization of SFB and prevents Th17-associated pathologies

Fig. 2

a DNA was extracted from the feces of Nfkbizfl/flVil1-Cre mice and co-housed gender-matched control mice (Nfkbizfl/fl). The amount of SFB was determined by qPCR, and shown as the SFB frequency among total eubacteria. The mean values are shown (n = 9 mice per group). b–d DNA was extracted from the indicated gastrointestinal regions of Nfkbizfl/flVil1-Cre and co-housed gender-matched control mice (Nfkbizfl/fl). The amount of SFB in each tissue was determined by qPCR and given after normalization to Actb (b). The mean values of frequency of SFB among total eubacteria (c) and the amount of Eubacteria in tissue (d) are shown (n = 9 mice per group). e The whole small intestine from the indicated mice was fixed, and embedded after preparation of the “Swiss roll”. The cryo-section of the intestine was analyzed by in situ hybridization using the indicated probes and staining with HoechstⓇ33342 (n = 5–6 mice per group). Scale bar, 1 mm. f The magnified images of the boxed area in (e) are shown. Scale bar, 100 μm. g The intestinal region was defined by the ratio of the florescent intensity of Lct to that of Slc10a2, and the signals for SFB in each region in (f) were quantified. The mean signal intensities are shown (n = 5–6 mice per group). Statistical significance was determined by Mann–Whitney U test. *p < 0.05, **p < 0.01, ***p < 0.001, n.s. not significant.

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