Fig. 3: Lack of IκBζ in IECs causes drastic alteration in the microbiota in the small intestine. | Mucosal Immunology

Fig. 3: Lack of IκBζ in IECs causes drastic alteration in the microbiota in the small intestine.

From: IκBζ controls IL-17-triggered gene expression program in intestinal epithelial cells that restricts colonization of SFB and prevents Th17-associated pathologies

Fig. 3

Bacterial composition of the jejunum, upper ileum, or feces from the indicated mice was investigated by sequencing the v4 region of the 16 S rRNA gene (n = 9 mice per group). a–c α-diversity based on the Shannon index (a) OTU richness (b), and Shannon evenness (c) was examined. The mean values are shown. Statistical significance was determined by Mann–Whitney U test. d, e β-diversity between control (Nfkbizfl/fl) and Nfkbizfl/flVil1-Cre mice was examined. Bray–Curtis dissimilarity indices of microbiota within each group and that between the two groups are shown as the box plot, and statistical significance was determined by Kruskal–Wallis test followed by Dunn’s multiple comparisons test (d). P values in the non-metric multi-dimensional scaling (NMDS) plot were obtained using PERMANOVA (e). f Linear discriminant analysis effect size (LEfSe) was analyzed. Differentially abundant operational taxonomic units (OTUs) are shown with linear discriminant analysis (LDA) values in LEfSe in accordance with the criteria of p < 0.05, positive false discovery rate (FDR) < 0.05, and modulus of signal-to-noise ratio (|SNR | ) > 0.5. g The composition of taxonomic families and orders was analyzed. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, n.s., not significant.

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