Fig. 6: Deletion of IκBζ in IECs leads to decrease in Paneth cells. | Mucosal Immunology

Fig. 6: Deletion of IκBζ in IECs leads to decrease in Paneth cells.

From: IκBζ controls IL-17-triggered gene expression program in intestinal epithelial cells that restricts colonization of SFB and prevents Th17-associated pathologies

Fig. 6

a Small intestinal organoids from wild-type mice were stimulated with IL-17A (20 ng/ml) for the indicated periods. Expression of the indicated genes was analyzed by RT-qPCR. The results are presented as the mean ± SD of triplicates and are representative of organoids from three mice. b Organoids from control (Nfkbizfl/fl) or Nfkbizfl/flVil1-Cre mice were unstimulated or stimulated with IL-17A for 24 h and the expression of the indicated genes was analyzed as in (a). c, d Tissue sections of the indicated regions of the small intestine were stained with hematoxylin-eosin (H&E) or anti-lysozyme antibody. Scale bars, 50 μm (c). e, f Tissue sections were stained with UEA-1, anti-E-cadherin antibody, and Hoechst 33258. Scale bars, 25 μm. (e). Magnified images of the box are shown to the bottom. Results are representative of six independent experiments (c, e). Lysozyme+ or UEA-1+ cells were counted in 50 crypts per region per mouse (n = 6 mice per group), and are shown as the mean number per crypt (d, f). Statistical significance was determined by the Mann–Whitney U test. *p < 0.05, **p < 0.01.

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