Fig. 9: NF-κB p50 is required for IκBζ-mediated gene regulation in IECs. | Mucosal Immunology

Fig. 9: NF-κB p50 is required for IκBζ-mediated gene regulation in IECs.

From: IκBζ controls IL-17-triggered gene expression program in intestinal epithelial cells that restricts colonization of SFB and prevents Th17-associated pathologies

Fig. 9

a The small intestinal organoids from control (Nfkb1+/−) or NF-κB p50-deficient (Nfkb1−/−) mice were unstimulated or stimulated with IL-17A (20 ng/ml) for 24 h. Expression of the indicated genes was analyzed by RT-qPCR. The results are presented as the mean ± SD of triplicates and are representative of organoids from three mice. b Organoids from the indicated mice were treated with IFN-γ (20 ng/ml), washed out, and re-cultured in fresh media for 4 days in the absence or presence of IL-17A (20 ng/ml). Expression of the indicated genes was analyzed as in (a). c, d Total RNA was extracted from the indicated intestinal regions (c) or the ileum (d) of the indicated mice. Expression of the indicated genes was analyzed by RT-qPCR. The mean expression levels are shown (n = 6 mice per group). Statistical significance was determined by Mann–Whitney U test (c, d). *p < 0.05, **p < 0.01, ****p < 0.0001, n.s., not significant.

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