Fig. 2: Global map of immune cells in lung revealed by the scRNA-seq analysis in mouse models of asthma exacerbation.

Droplet-based single-cell (sc)-RNA sequencing was performed on CD45⁺ single-cell suspensions pooled from six lungs per group including saline, HDM/control (CTRL), HDM/HDM (H/HDM), HDM/CRE (H/CRE), and HDM/RSV (H/RSV) treated animals. All samples were analyzed using canonical correlation analysis with the Seurat R package. Cells were clustered using a graph-based shared nearest-neighbor clustering approach and plotted by a Uniform Manifold Approximation and Projection (UMAP) for Dimension Reduction. a Violin plot of expression levels of classical immune cell surface markers found in the CD45⁺ cells in the lung, correlated to relevant immune cluster (clusters 0–25). b Heatmap representation of annotation score of immune cell clusters with the expression, by SingleR R package. c Graphical representation of 26 major immune clusters identified by scRNA sequencing, represented using UMAP analysis. d Dot plot of expression levels of inflammatory factors within identified immune clusters.