Fig. 4: Flow cytometry validates unique Mφ subpopulations during muscularis inflammation. | Mucosal Immunology

Fig. 4: Flow cytometry validates unique Mφ subpopulations during muscularis inflammation.

From: Enteric glial cells favor accumulation of anti-inflammatory macrophages during the resolution of muscularis inflammation

Fig. 4

a–h from naïve CX3CR1GFP/+ mice, 24 h and 72 h after muscularis inflammation. a Contour plots representing CX3CR1 expression in Ly6C− MHCIIhi Mφs (left). Percentages and absolute numbers of CX3CR1hi and CX3CR1lo cells from Ly6C− MHCIIhi Mφs are shown as mean ± SEM (right). b Contour plots representing CX3CR1 expression in Ly6C− MHCIIlo Mφs (left). Percentages and absolute numbers of CX3CR1hi and CX3CR1lo cells from Ly6C− MHCIIlo Mφs are shown as mean ± SEM (right). c–h CD206 and CCR2 expression in Ly6C− MHCIIhi and MHCIIlo Mφs. Contour plots representing CD206 or CCR2 expression in Ly6C− MHCIIhi (c) and Ly6C− MHCIIlo Mφs (f). Percentages of CD206+ CX3CR1hi, CD206− CX3CR1hi and CD206+ CX3CR1lo cells in the LY6C− MHCIIhi Mφs (d) and MHCIIlo Mφs (g). Percentages of CCR2+ CX3CR1hi, CCR2− CX3CR1hi and CCR2+ CX3CR1lo cells in the LY6C− MHCIIhi Mφs (e) and MHCIIlo Mφs (h). One-way ANOVA; test *p < 0.05; **p < 0.01; ns not significant.

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