Fig. 2: Multimerization of S40-based carriers. | Mucosal Immunology

Fig. 2: Multimerization of S40-based carriers.

From: A lentiviral vector expressing a dendritic cell-targeting multimer induces mucosal anti-mycobacterial CD4+ T-cell immunity

Fig. 2

a Western-blot analysis of supernatants and cell lysates of HEK293T cells, either transduced at an MOI = 100 of LV::S40-H-StrepII or non-transduced, in reducing or non-reducing conditions. β-Actin staining was use of as loading control. b Chromatogram of column calibration using a set of purified recombinant proteins on a Superose 6 FPLC column. X axis correspond to MW equivalence observed during elution and Y axis to UV absorbance (280 nm). c Chromatogram of 50x-concentrated supernatant of HEK293T cells transduced with LV::S40-H-StrepII. d Quantification of S40-H-StrepII protein by measuring mean pixel intensity of the band of each well, corresponding to the molecular weight of monomeric S40-H-StrepII. e Western blot analysis of elution fractions in reducing conditions.

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