Fig. 4: Phenotypic Maturation of DCs Induced by M40 or S40. | Mucosal Immunology

Fig. 4: Phenotypic Maturation of DCs Induced by M40 or S40.

From: A lentiviral vector expressing a dendritic cell-targeting multimer induces mucosal anti-mycobacterial CD4+ T-cell immunity

Fig. 4

a Phenotypic maturation of BM-DCs from C57BL/6 mice infected at an MOI of 5 with Mtb, as a positive control, or incubated with supernatants from HEK-293T cells transduced (MOI = 20) with LV::EsxH alone (Ctrl), LV::M40-H, or LV::S40-H. Expression of co-stimulatory or MHC molecules on the surface of CD11b+ CD11c+ cells at 24 h post-immunization was assessed by cytometry. b Heatmaps showing the mean fluorescence intensity (MFI) of CD40 and CD80 surface expression and the percentage of CD86hi, MHC-I hi, and MHC-II hi DCs. c Quantitation of inflammatory mediators in the culture supernatants of the same DCs. Results are representative of at least two independent experiments.

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