Fig. 5: Immunogenicity of Multi-Antigenic LV::S40-HAPEHR or LV::S40-HAPEHR-20. | Mucosal Immunology

Fig. 5: Immunogenicity of Multi-Antigenic LV::S40-HAPEHR or LV::S40-HAPEHR-20.

From: A lentiviral vector expressing a dendritic cell-targeting multimer induces mucosal anti-mycobacterial CD4+ T-cell immunity

Fig. 5

a IFN-γ T-cell responses, as assessed by ELISPOT on day 13 post-immunization, in the spleens of individual C57BL/6 mice (n = 3) immunized s.c. with 1 × 108 TU/mouse of LV::S40-HAPEHR or LV::S40-HAPEHR-20. The frequency of responding T cells was determined following in vitro stimulation with the indicated synthetic peptides. Quantitative differences between the groups of mice immunized with LV::S40-HAPEHR or LV::S40-HAPEHR-20, were not statistically significant (Mann–Whitney test). b Epitope mapping of Hrp-1 and RfpD, as determined using pooled splenocytes from 3 C57BL/6 mice/group injected with PBS or immunized s.c. with 1 × 108 TU/mouse of LV::S40-HAPEHR prior to stimulation with each of the individual peptides from the Hrp-1- or RpfD-derived overlapping 15-mers offset by five amino acids. c Cytometric analysis of intracellular IFN-γ vs IL-2 staining of CD4+ T splenocytes after stimulation with 10 µg/ml of the indicated peptides encompassing the immunodominant epitopes identified in b. Pooled splenocytes from three mice/group were used.

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