Fig. 1: Vaccination with C. neoformans Δsgl1 drives T cell polarization towards a protective type 1/17 response in immunocompetent, CD4-deficient, and CD8-deficient mice.
From: Vaccine protection by Cryptococcus neoformans Δsgl1 is mediated by γδ T cells via TLR2 signaling
CBA/J mice (n = 6 mice/group/timepoint) were administered isotype (a–d), anti-CD8 (e, f), or anti-CD4 (g, h) antibodies. Mice then received either C. neoformans Δsgl1 (white symbols) or PBS (black symbols). After 30 days, mice were challenged with C. neoformans WT (day 0). On days −15, −1, 7, 15, and 24, mice were assessed for T cell-derived cytokines (IFNγ, IL-17A) via intracellular cytokine staining. Graphed data represents the mean ± SD from 2 independent experiments (n = 3 mice/group/timepoint for each biological replicate). Representative flow cytometry plots for days −1 and 7 for each group are shown, which were gated from live, CD45+ singlets (gating scheme shown in Supplementary Fig. 2). Significance was determined by a two-way ANOVA using Šídák’s multiple comparisons test for P value adjustment, and significance is denoted as *P < 0.05; **P < 0.01; ***P < 0.005; ****P < 0.001.
