Fig. 6: Toll-like receptor 2 (TLR2) is required in vivo for protective cytokine-producing γδ T cells to C. neoformans Δsgl1.

C57BL/6 (black symbols) or TLR2^−/− (white symbols) were administered C. neoformans Δsgl1 and assessed for T cell-derived cytokines via intracellular cytokine stimulation on days −30 (uninfected; a), −27 (b), and −23 (c). 30 days after C. neoformans Δsgl1 administration, mice were challenged with C. neoformans WT and assessed for T cell-derived cytokines via intracellular cytokine stimulation on days −1 (unchallenged; d), 3 (e), and 7 (f). At all timepoints, mice were assessed for the total number of γδ T, CD4⁺, and CD8⁺ T cells as well as the number of IFNγ- or IL-17A-producing subsets of these cells. Graphed data represent the mean ± SD and are representative of 1–2 independent experiments (n = 3–6 mice/timepoint/group). Significance was determined by a two-way ANOVA using Šídák’s multiple comparisons test for P value adjustment, and denoted as *P < 0.05, **P < 0.01, ***P < 0.005, ****P < 0.001.