Fig. 5: Neonates fail to upregulate antioxidants during influenza virus infection.
From: Severity of neonatal influenza infection is driven by type I interferon and oxidative stress
To determine changes in transcription of antioxidant enzymes in the infected animal, 3-day-old WT (black bars) and IFNαβR−/− (gray bars) neonatal and 8-week-old (white bars) mice were intranasally infected with PR8 influenza A virus. Lungs were harvested at 1- and 3-days post-infection, and from age-matched uninfected animals. a Sod3, b Gpx3, c Gss, and d Prdx1 transcriptions were normalized to the housekeeping gene gapdh (n = 3–6 for each group). Data presented relative to uninfected age-matched mice. Data from 3 independent experiments. Statistical differences between groups were assessed sing one-way ANOVA to compare multiple groups, where denoted ns = non-significant, *p < 0.05, **p < 0.01.
