Fig. 1: Factors critical for intestinal epithelial function exhibited similar protein expression changes and factors associated with T cell function, but not macrophage function, exhibited similar gene expression changes between C. rodentium-induced colitis and human IBD tissues.
a FVB mice were infected with C. rodentium p.o. and disease progression was measured using endoscopy at various times p.i. (n = 5–9 mice per time point). b Volcano plot depicting protein expression changes on day 8 p.i. compared to naïve mice detected using MS based proteomics. c Heat map of select protein expression changes in infected FVB mice with previously published CD or UC proteomics datasets20, compared to respective healthy controls. d IPA pathway, upstream regulator and function comparisons between mouse cell Cluster 4 and a human highly activated T cell cluster. e IPA pathway, upstream regulator and function comparisons between mouse cell Cluster 3 and a human inflammatory macrophage cluster. *p < 0.05, ***p < 0.001 determined as follows: Kruskal–Wallis with Dunnett’s multiple comparisons test (a). Data are representative of five independent experiments (a). Horizontal bars represent the mean of the points shown (a).
