Fig. 3: Heterogeneity of intestinal epithelial cells in C. rodentium induced colitis. | Mucosal Immunology

Fig. 3: Heterogeneity of intestinal epithelial cells in C. rodentium induced colitis.

From: Epithelial dysfunction is prevented by IL-22 treatment in a Citrobacter rodentium-induced colitis model that shares similarities with inflammatory bowel disease

Fig. 3

CITE-seq was conducted on lamina propria cells isolated from various treatment groups – infected (untreated, vehicle or IL-22.Fc treated) or naïve mice (n = 5 mice per group). a Integrated UMAP clustering of all different treatments highlighting the epithelial cell clusters that can be distinguished in the feature plot by expression of EPCAM antibody derived tag (inset). Clusters 0, 12, 16, 22 and 23: epithelial cells; Clusters 1, 3 and 9: macrophages/monocytes; Clusters 2, 8, 11 and 19: dendritic cells; Cluster 4: T cells, NKT cells and innate lymphoid cells; Clusters 5, 6 and 14: B cells; Clusters 7 and 10: fibroblasts; Clusters 15 and 21: endothelial cells; Cluster 17: stromal cells; Clusters 13, 18 and 20 contained mixed populations. b Pseudotime trajectory analysis of the IECs with stem cells (white), intermediate cells (Branched [B] or transitional [T]; grey), colonocytes (green), and goblet cells/EECs (blue). c Distribution of IEC types identified using pseudotime trajectory analysis in the various treatment groups.

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