Fig. 4

Impaired social interactions, though not nest building in Fmr1^KO mice are improved by GSK6A. a In a three-chamber social interaction assay, vehicle-treated WT mice spent more time in a chamber associated with a conspecific than an object, whereas vehicle-treated Fmr1^KO mice did not show a preference. In contrast, GSK6A-treated Fmr1^KO mice preferred the “social chamber” (three-way repeated-measures ANOVA: p(drug × genotype × chamber) = 0.018, F(1,50) = 6; *p = 0.037, ^#p = 0.008). GSK6A treatment abolished social preference in WT mice. b–d Nest scores were lower (b) and percent torn nestlet was lower (c, d) in Fmr1^KO mice. One or two consecutive daily injection(s) of GSK6A were ineffective in improving nest scores (two-way ANOVAs with Tukey’s post hoc tests: one injection (b): p(drug) = 0.28, F(1,57) = 1.2; p(genotype) = 0.0002, F(1,57) = 15.5; p(interaction) = 0.27, F(1,57) = 1.3; *p = 0.004; ^#p = 0.005; p(ko/veh-ko/6A) > 0.999; two injections (data not shown): p(drug) = 0.59, F(1,39) = 0.3; p(genotype) < 0.0001, F(1,39) = 20.3; p(interaction) = 0.40, F(1,39) = 0.7; p(wt/veh-ko/veh) = 0.003; p(wt/veh-ko/6A) = 0.005; p(ko/veh-ko/6A) = 0.996) or improving percent nestlet torn (two-way ANOVAs with Tukey’s post hoc tests; one injection (c): p(drug) = 0.014, F(1,57) = 6.5; p(genotype) = 0.0007, F(1,57) = 12.7; p(interaction) = 0.676, F(1,57) = 0.18; *p = 0.031; ^#p = 0.0004; p(ko/veh-ko/6A) = 0.44; two injections (d): p(drug) = 0.99, F(1,39) = 0.0001; p(genotype) < 0.0001, F(1,39) = 20.8; p(interaction) = 0.82, F(1,39) = 0.1; *p = 0.008; ^#p = 0.012; p(ko/veh-ko/6A) = 0.998). Representative images of nests are shown in Fig. S6f