Fig. 5
Adolescent CIE-induced anxiety-like behavior can be blocked by SK channel agonist 1-EBIO and mimicked by SK channel antagonist apamin microinjected into the NAcS. a Experimental timeline. b Example LDT maps in the light compartment, surrounded by black lines (indicating the walls of light compartment) and white bars (indicating the tunnel to the dark compartment) from rats with a history of CIW vs. CIE during their adolescent stage, treated with microinjections of ACSF vs. 1-EBIO (324 ng in 0.5 μL per injection site at a concentration of 4 mM) in the NAcS 10 min before test. c Summarized results showing 1-EBIO specifically increased the crossover latency in rats with a history of adolescent CIE treatment, by which their latency was reversed back to a similar level as in the CIW control group (Ado::CIW/Ado::CIE × ACSF/1-EBIO interaction F1,40 = 6.1, p = 0.02). d Example LDT maps from rats with a history of CIW vs. CIE during their adolescent stage, treated with microinjections of ACSF vs. apamin (2 ng in 0.5 μL, per injection site at a concentration of 2 μM) in the NAcS 10 min before the test. e Summarized results showing apamin specifically decreased the crossover latency in rats with a history of adolescent CIW treatment, by which their latency reaches a level similar to the adolescent CIE group Ado::CIW/Ado::CIE × ACSF/apamin interaction F1,34 = 12.6, p < 0.01. f, g Summarized results showing that, compared with ACSF, neither 1-EBIO nor apamin affected the speed (f, Ado::CIW/Ado::CIE × ACSF/1-EBIO interaction F2,68 = 0.62, p = 0.55) or the total distance traveled (g, Ado::CIW/Ado::CIE × ACSF/1-EBIO/apamin interaction F2,68 = 0.61, p = 0.54) during the OF test. h Diagrams of coronal slices showing the cannulation sites in the NAcS from rats used for LDT test. The animal number (i.e., n) is shown in parentheses for each group. Data were analyzed by two-way ANOVA, followed by Bonferroni post test. *p < 0.05
