Fig. 1: Reduced firing activity of PNs in the BTBR cerebellum. | Neuropsychopharmacology

Fig. 1: Reduced firing activity of PNs in the BTBR cerebellum.

From: Targeting inhibitory cerebellar circuitry to alleviate behavioral deficits in a mouse model for studying idiopathic autism

Fig. 1

a Schematics of cerebellar circuitry including excitatory and inhibitory inputs to a PN. BC basket cell, SC stellate cell, DCN deep cerebellar nuclei, CF climbing fiber, MF mossy fiber, PF parallel fiber, GC granule cell. (b, c Representative action currents (i.e., APs in the voltage-clamp mode) from PNs recorded in the cell-attached configuration from WT (b) and BTBR (c) brain slices in the absence (control, top panels) or presence of AMPA-receptor and NMDA-receptor blockers NBQX (10 μM) and APV (50 μM) (middle panels), or in a combination of NBQX, APV, and a GABAA receptor blocker bicuculline (10 μM, bottom panels). d Summary of frequency of APs from WT (black bars, n = 10) and BTBR (blue bars, n = 10) PNs in the aforementioned conditions (b, c). The frequency is the reciprocal of each inter-AP-intervals. e Summarized coefficient of variation (CV) of inter-AP-intervals for the same WT (black bars, n = 10) and BTBR (blue bars, n = 10) neurons. # indicates significant differences between “NBQX + APV + bicuculline” and “control” conditions within each group. Data are represented as mean ± s.e.m. Asterisks (*) or “ns” denotes statistical significance (p < 0.05) or “not significant” respectively in this and following figures.

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