Fig. 4: Global alterations in gene expression in the mPFC of ELS mice.

a Schematic representation of the experimental procedure of mPFC RNAseq data collection. b Global DNA methylation levels are not significantly altered in the mPFC of adult male mice; CTR n = 7, ELS n = 9. c Normalized trace representation of fold change (log₂ on y axis) and q value (log₁₀ on x axis) for all transcripts detected in the RNAseq in CTR and ELS animals (total of 13,865 transcripts); significantly altered transcripts are represented in purple as q < 0.05 in FDR adjusted p values; CTR n = 3, ELS n = 3. d Circos plot display of upregulated transcripts in red (total of 78) and downregulated transcripts in blue (total of 102) in ELS mice compared with CTR; solid bars represent fold change (log₂) and transparent portion of each bar represents transcript abundance in Fragments Per Kilobase Million (FPKM in log₁₀). e Chord diagram highlighting genes belonging to the Gene Ontology categories, “Biological Process—Behavior,” “Cellular component—Synapse,” and “Molecular Function—Receptor Activity”; genes belonging to all three categories are connected by gray cords. f Validation by qRT-PCR of receptor channels involved in behavioral regulation; CTR n = 5–13, ELS n = 6–18. Statistical significance was set at, *p < 0.05 with FDR correction. Data in (b) and (f) are presented as means ± SEM.