Fig. 5: A comparison of complex behavioral scoring between human raters, machine learning classifiers and commercially available solutions.

a Schematic of the workflow. b, c Unsupported and supported rears in the open field test as reported by three human raters (averaged and plotted as manual scoring) and three machine learning classifiers (averaged and plotted as ML classifiers), EthoVision XT14 and the TSE Multi Conditioning System (TSE). d, e Correlation analysis for comparison. Data expressed as mean ± standard error of the mean. Colors represent individual animals and are consistent across analysis techniques for comparison (n = 20). *p < 0.05, **p > 0.01, ***p < 0.001, ****p > 0.0001.